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  1. Products
  2. Low Endotoxin Solutions
  3. Arcofeliz ™ EN series: Low-Endotoxin Enzyme
Arcofeliz ™

EN series: Low-Endotoxin Enzyme

The Arcofeliz™ EN series provides high-performance, low-endotoxin enzymatic solutions specifically designed for advanced bioscientific applications. These enzymes are produced through highly controlled fermentation processes and multi-step purification to ensure the highest level of biological safety.

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Chemical Name:

Alginate lyase (Low Endotoxin Grade)
Collagenase (Low Endotoxin Grade)

Applications & Uses:

  • Cell Recovery & Isolation

Arcofeliz™ EN-ALGS (Alginate Lyase): Facilitates the gentle recovery of encapsulated cells by liquefying alginate hydrogels under physiological conditions.
Arcofeliz™ EN-COLG (Collagenase): Ideal for dissociating tissues and isolating cells from collagen-rich extracellular matrices.


  • Regenerative Medicine: Both enzymes are refined to minimize unintended immune responses, making them suitable for sensitive scaffold depolymerization and tissue engineering.
  • Hydrogel Liquefaction: Rapidly reduces the viscosity of biomaterial matrices while maintaining high cell viability and functional integrity.
FAQ

Benefits:

  • Physiological Compatibility: Arcofeliz™ EN maintains over 60% relative activity at neutral pH, allowing for mild treatment in cell culture environments.
  • High Purity & Safety: Advanced chromatographic purification removes non-specific proteolytic impurities and ensures ultra-low endotoxin levels.

Performance Attributes:

SourceProductSpecifications
Alginate LyaseArcofeliz™ EN-ALGSEnzymatic Activity
Endotoxin Level
≥20,000 units/g
≤10,000 EU/g
CollagenaseArcofeliz™ EN-COLGEnzymatic Activity
Endotoxin Level
≥20,000 units/g
≤10,000 EU/g

Approvals & Certifications:

ISO9001

Brand Description:

Arcofeliz™ EN series represents Nagase ChemteX’s dedication to providing high-purity enzymatic solutions for the next generation of healthcare. By combining advanced microbial fermentation with sophisticated purification techniques, we deliver Alginate Lyase and Collagenase that maintain maximum functional activity while meeting the stringent safety requirements of bioscientific research and clinical applications.

Manufacturer:

Nagase ChemteX Corporation

Industries:

  • Healthcare & Pharmaceuticals

Categories:

  • Gelling Agents
  • Low Endotoxin

Country of origin:

  • JP

Physical Properties:

Appearance: Alginate Lyase: A globular protein produced through specialized microbial fermentation.
Collagenase: Supplied as a high-purity lyophilized powder that dissolves clearly in neutral buffers (e.g., PBS or Tris-HCl).
Solubility: Alginate Lyase & Collagenase: Both provide excellent solubility.

Chemical Properties:

Structure: Alginate Lyase: Characterized by a specialized active site cleft, typically featuring an (α/α)_6 toroid or β-jellyroll fold.
Collagenase: Comprises a catalytic domain, a linker region, and collagen-binding domains (CBDs).
Purity: Alginate Lyase & Collagenase: Both provide strictly controlled endotoxin levels (≤ 10,000 EU/g)

Mechanical Properties:

Substrate Specificity: Alginate Lyase & Collagenase: Both are highly effective in degrading their respective target substrates.
Enzymatic Potency: Alginate Lyase & Collagenase: Both provide high specific activity (≧20,000 units/g) for effective tissue dissociation.

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© {{lastPublishedYear}} Nagase & Co., Ltd. All rights reserved.