Molecular Diagnostic Enzymes

Applications & Uses:

• Standard and real-time quantitative PCR (qPCR) assays
• One-step and two-step RT-qPCR pathogen detection
• Point-of-Care (POC) isothermal gene amplification (LAMP) assays
• Direct amplification from unpurified clinical samples such as whole blood, saliva, and urine
• Methylation-specific PCR (qMSP) and gene mutation detection (ARMS-PCR)

Benefits:

• Direct PCR Capability & Resistance to Whole Blood: Enables amplification directly from whole blood samples without prior DNA purification.
• Rapid Amplification Speed: Features fast extension rates, shortening reaction times and overall diagnostic turnaround.
• High Sensitivity & Reproducibility: Delivers clear amplification and reliable detection performance, even for low-copy gene templates.
• Robust Inhibitor Tolerance: Tolerates various PCR inhibitors present in complex clinical and environmental samples, such as heparin, hematin, humic acid, and crude extracts.
• Consistent Lot-to-Lot Quality: Maintains uniform functional activity across production batches to support reproducible results.

Performance Attributes:

• Display extension speed up to 7.5 seconds/kb under standard molecular assays.
• Demonstrate stable performance with 37°C accelerated stability over 21 days and stable storage at 2-8°C for over 3 years.
• Withstand up to 10 freeze-thaw cycles without significant loss of enzymatic activity.

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