Recombinant BSA (rBSA)
A molecular biology and diagnostic-grade Recombinant Bovine Serum Albumin (rBSA) expressed heterologously in yeast cells. Highly purified and 100% animal-free, it is designed to replace native, animal-derived BSA in immunoassays and molecular diagnostics. Due to its high blocking and stabilizing efficiency, it allows for lower working concentrations than standard bovine-derived BSA, reducing background noise and supporting assay sensitivity.
Applications & Uses:
- Surface blocking in ELISA, CLIA, western blotting, and lateral flow assays
- Liquid-phase stabilization of enzyme-labeled conjugates (HRP, AP) and antibodies
- Nuclease-free protein carrier and protector in PCR, qPCR, and RT-qPCR assays
- Prevention of enzyme adhesion to pipettes and reaction tube walls
| Grade | Target Application | Quality Standards & Physicochemical Specifications | Recommended Uses & Performance Attributes |
|---|---|---|---|
| rBSA HC / Immunoassay | Immunoassay Grade |
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| rBSA HA / Molecular Biology | Molecular Biology Grade |
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Benefits:
- Animal-origin-free manufacturing: Produced using a yeast expression system without animal-derived ingredients.
- High blocking efficiency: Under comparative test conditions, 1g of lyophilized rBSA provides blocking performance equivalent to 20g to 100g of native bovine-derived BSA.
- Nuclease & DNA-free: Certified free of detectable DNase, RNase, and host E. coli DNA (Cq > 35), preventing background interference in molecular assays.
Performance Attributes:
- Demonstrates 20 to 100 times higher blocking efficiency compared to native BSA under comparative test conditions.
- Stabilizes proteins and maintains a clean diagnostic baseline free of detectable nuclease or host DNA background.
Sub Category:
Blocking ReagentsPhysical Properties:
| Appearance: | White to off-white lyophilized powder (solid) |
|---|---|
| Solubility: | Readily and fully soluble in PBS and standard reconstitution buffers |
| Molecular Weight: | ~67,205 Daltons |
Chemical Properties:
| Extinction Coefficient: | 42,925 at 280 nm |
|---|---|
| Concentration: | Lyophilized 100% active protein (glycerol-free and salt-free) |
| pH stability: | Highly stable across broad assay reaction pH |
| Preservatives: | None |
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