Recombinant HSA (rHSA)
A diagnostic-grade Recombinant Human Serum Albumin (rHSA) expressed in rice endosperm. Completely animal-free, it mimics the physiological and structural properties of native human plasma albumin. Designed to replace native BSA or plasma-derived HSA (pHSA), it serves as a stabilizer, blocker, or serum matrix component in CLIA, ELISA, and mass spectrometry, reducing blood component interference and IgG-induced false positives.
Applications & Uses:
- Enzyme-labeled antibody stabilization and buffer formulation in CLIA and ELISA
- Hormone-free and lipid-free serum matrix control for Mass Spectrometry (LC-MS/MS) and chromatography
- Surface blocking in clinical assays to reduce IgG-induced cross-reactivity
- Xeno-free and animal-free cell culture media supplement for gene therapy, vaccine production, and cryopreservation
| Grade | Endotoxin Limit | Target Applications & Assay Performance |
|---|---|---|
| Premium Grade | < 0.01 EU/mg (Liquid format) |
|
| Advanced Grade | < 0.125 EU/mg (Lyophilized powder) |
|
| Precise Grade | Managed by specific assay |
|
Benefits:
- Animal-origin-free manufacturing: Produced in rice endosperm without animal-derived ingredients.
- Zero IgG contamination: Completely free of bovine and human IgG residues, preventing cross-reactive false positives and assay retests.
- High physicochemical purity: Monomer purity is equal to or greater than 99.0% with zero post-translational glycosylation, delivering molecular consistency.
- Low analytical background: Lacks endogenous lipids, steroids, or hormones, making it ideal for clean, high-sensitivity mass spectrometry baselines.
Performance Attributes:
- Maintains acridinium-based chemiluminescent signal stability (demonstrating a minimal 8-day RLU deviation of plus 2.1% at 37°C).
- Provides protein protection and reduces cross-reactivity in sensitive assay systems.
Sub Category:
Blocking ReagentsPhysical Properties:
| Appearance: | White to off-white lyophilized powder (solid) or clear colorless liquid (premium liquid grade) |
|---|---|
| Solubility: | Readily and fully soluble in PBS and water |
| Molecular Weight: | ~66,550 Daltons |
Chemical Properties:
| Glycosylation: | No glycosylation detected |
|---|---|
| Free thiols content: | ~89% |
| Isoelectric Point (pI): | 4.8 |
| Formulation: | Free of detergents (no Tween-80) and animal-derived additives |
| Preservatives: | None |
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