Bis Tris
A highly specialized, GMP-grade zwitterionic biological buffer system engineered for molecular biology, gel electrophoresis, and diagnostic assay kits. Sourced through a rigorous GMP manufacturing process, Bis Tris is specifically optimized for slightly acidic to neutral environments (pH 5.8 to 7.2). Compared with standard Tris, it exhibits significantly lower temperature sensitivity and a superior ability to stabilize enzyme and protein structures under analytical thermal stress.
Chemical Name:
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methaneApplications & Uses:
- Gel electrophoresis running buffer and gel preparation (especially for Bis-Tris PAGE of proteins)
- Buffering agent for diagnostic assay kits requiring slightly acidic environments
- Stabilization and protection of enzymes and antibody-conjugates during diagnostic assays
- Chromatography mobile phase and biochemical sample reconstitution buffer
Benefits:
- Specialized for slightly acidic to neutral environments with outstanding buffering stability
- Lower sensitivity to temperature fluctuations compared with standard Tris buffers
- Stronger ability to react with and stabilize systems containing heavy metal ions
- GMP-level manufacturing process enables rigorous control of microbial and endotoxin levels
- Features a highly stable crystalline form, low endotoxins, and extremely high assay purity of 99.5% or more
Performance Attributes:
- Displays outstanding buffering capacity within pH 5.8 to 7.2 with high ionic reproducibility.
- Strongly suppresses non-specific protein precipitation and aggregation under thermal stress.
- Maintains high pH stability in slightly acidic reaction environments.
Approvals & Certifications:
REACH, CDE, CMDE, and China Cosmetic Ingredient Safety Information registrationsSub Category:
Biological BuffersPhysical Properties:
| Appearance: | White crystalline powder |
|---|---|
| Solubility: | Soluble in water |
| Crystalline stability: | High |
Chemical Properties:
| Assay: | Equal to or greater than 99.5% |
|---|---|
| pH Range: | 5.8 to 7.2 |
| Endotoxin Level: | Low endotoxin (rigorously controlled) |
| Microbial count: | Minimal |
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