Trehalose Enhances Removal of Host Cell–Derived Impurities During Antibody Harvest
March 30, 2026
This webinar provides in-depth technical insights into how trehalose supplementation during antibody harvesting can reduce host cell–derived impurities and enhance downstream purification performance.
Presentation Title:
Trehalose Enhances Removal of Host Cell-derived Impurities During Harvesting Process of Antibody Production
Presentation Overview:
In monoclonal antibody production, host cell protein (HCP) carryover from the harvest step can significantly impact downstream purification efficiency and Protein A performance.
In the webinar, Kotaro Hashimoto, Senior Scientist in Nagase Viita Co., Ltd., presents data showing how adding trehalose during CHO cell harvest can:
- Reduce HCP levels in the clarified filtrate
- Lower supernatant conductivity
- Improve clarification performance
- Increase antibody recovery during Protein A purification
The study was conducted with chromatographic clarification using the 3M™ Harvest RC system. This approach offers a novel and practical strategy to minimize impurity carryover into downstream purification, improving overall process efficiency and antibody yield.
Presentation Overview:
In monoclonal antibody production, host cell protein (HCP) carryover from the harvest step can significantly impact downstream purification efficiency and Protein A performance.
In the webinar, Kotaro Hashimoto, Senior Scientist in Nagase Viita Co., Ltd., presents data showing how adding trehalose during CHO cell harvest can:
- Reduce HCP levels in the clarified filtrate
- Lower supernatant conductivity
- Improve clarification performance
- Increase antibody recovery during Protein A purification
The study was conducted with chromatographic clarification using the 3M™ Harvest RC system. This approach offers a novel and practical strategy to minimize impurity carryover into downstream purification, improving overall process efficiency and antibody yield.